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TIME magazine named this the second most important discovery that year. While iPS cells appear to have reverted back to embryonic childhood, Ryan found they carry some adult baggage with them, retaining chemical memories. These memories may result in unpredictable and undesirable cell growth, limiting medical potential of iPS cells.

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The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. In a hematopoiesis radiation recovery assay, transplantation of GFP-labeled marrow cells allowed for striking in vivo visual assessment of engraftment from 2 hours-5 weeks post transplant. Using FACS analysis, both transparent and wild-type fish had equal engraftment, but this could only be visualized in the transparent recipient. In a tumor engraftment model, transplantation of RAS-melanoma cells allowed for visualization of tumor engraftment, proliferation and distant metastases in as little as 5 days, which is not seen in wild-type recipients until weeks.


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