Sperm subpopulation rat velocity

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Skip to search form Skip to main content. Quantification and identification of sperm subpopulations using computer-aided sperm analysis and species-specific cut-off values for swimming speed. Motility is an essential characteristic of all flagellated spermatozoa and assessment of this parameter is one criterion for most semen or sperm evaluations.

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E Corresponding author. Email: daznia. For over 30 years, CASA-Mot technology has been used for kinematic analysis of sperm motility in different mammalian species, but insufficient attention has been paid to the technical limitations of commercial computer-aided sperm analysis CASA systems.

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Abstract Here we show the effects of repeated electroejaculation EE on mean values of motility, mitochondrial functionality, and expression of active caspases on goat sperm obtained by EE. Evaluations were done using CASA and flow cytometry. A strategy for identification of kinematic sperm subpopulations, when individual data of sperm are not provided by the CASA system, is provided. Fifty semen samples, five of each of ten adult creole goats, were obtained by electroejaculation.

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The present study was undertaken to investigate the effects of freezing on sperm velocity in four different age groups young: tender TCAYE was added to semen samples and cryopreserved using a programmable freezer prior to plunging into liquid nitrogen oC. The fresh extended and frozen-thawed semen were subjected to computer automated semen analyzer CASA for sperm velocity evaluation. Fresh extended sperm in mature age group showed significantly highest values for average path velocity VAP

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The effect of inhaled epichlorohydrin on rat sperm motility characteristics was evaluated. Male F rats were exposed to ppm epichlorhydrin via inhalation for 4 hr in the morning of Day 0 and killed immediately and on Days 1, 2, 6, and 14 postexposure. Epichlorohydrin did not affect the percentage of motile sperm at any time.

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Computer-aided sperm analysis CASA technology was developed in the late s for analyzing sperm movement characteristics or kinematics and has been highly successful in enabling this field of research. CASA has also been used with great success for measuring semen characteristics such as sperm concentration and proportions of progressive motility in many animal species, including wide application in domesticated animal production laboratories and reproductive toxicology. However, attempts to use CASA for human clinical semen analysis have largely met with poor success due to the inherent difficulties presented by many human semen samples caused by sperm clumping and heavy background debris that, until now, have precluded accurate digital image analysis. The authors review the improved capabilities of two modern CASA platforms Hamilton Thorne CASA-II and Microptic SCA6 and consider their current and future applications with particular reference to directing our focus towards using this technology to assess functional rather than simple descriptive characteristics of spermatozoa.

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Feline semen evaluation is basically performed for commercial, diagnostic, and research purposes. Several studies have highlighted, in the raw ejaculate, the presence of different subpopulations classified based on specific characteristics, such as morphology Nunez-Martinez et al. However, the sperm kinetic parameters are the most-used features to identify sperm subpopulations in humans Chantler et al.

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